Protein Standard Separation

• Packing: Jordi Hydroxylated Polyamide
• Column: 4.6 X 50mm ID
• Flow Rate: 1.0 mL/min
• Temp.: Ambient
• Detection: UV @ 220nm 0.5 AUFS
• Injection: 40uL Protein Stds. Mix Sigma PN H-2899
• Concentration: Protein Stds Diluted to 4mL in 75/25 A/B
• Solvent: 80/20 to 40/60 A/B 30 min linear A: 0.15% TFA in H2O B: 0.15% TFA in ACN